Transcriptome analysis of oral bacterial pathogens
We are analyzing the effects of growth as a bacterial biofilm and the growth in a polymicrobial biofilm on gene expression by Porphyromonas gingivalis and Treponema denticola using DNA microarray analysis. These bacteria cause disease by growing as a consortium withTanerella forsythiain a polymicrobial biofilm. There is a degree of cooperativity between these bacteria that we are investigating by transcriptome analysis. These projects include the development of novel culture techniques for these Gram-negative obligate anaerobes.